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tcyJ [2018-05-04 13:04:02]
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tcyJ [2018-05-04 13:04:02]

cystine ABC transporter (binding protein)
locus
BSU29380
pI
9.60
mw
30.02 kDa
protein length
269 aa Sequence Blast
gene length
807 bp Sequence Blast
function
cystine uptake
product
cystine ABC transporter (binding protein)
essential
no
synonyms
ytmJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,007,426 → 3,008,235

    The protein

    Protein family

  • bacterial solute-binding protein 3 family (according to Swiss-Prot)

    • [SW|Localization]

  • attached to the membrane via [protein|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|TcyL]-[protein|B1DD32A3B3D1C0C5391A40834940257357A2F30F|TcyM] [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]
    description
    [Pubmed|15668000]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15272571], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16109943], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]
  • [protein|741156D495BE3857683C8A0390764EAD83845ABC|AscR]: activation, [Pubmed|16109943], in [regulon|741156D495BE3857683C8A0390764EAD83845ABC|AscR regulon]

    • regulation

  • induced in the presence of methionine and taurine [Pubmed|11390694]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A170 (ytmJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/170 NBRP B. subtilis, Japan]
  • 1A948 ( ''tcyJ''::''kan''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A948&Search=1A948 BGSC]
  • BKE29380 (Δ[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACGCTTATTCATAGGCTGCC, downstream forward: _UP4_TTTGAAAAATAAATGGGGCG
  • BKK29380 (Δ[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACGCTTATTCATAGGCTGCC, downstream forward: _UP4_TTTGAAAAATAAATGGGGCG

    • References

  • 15262924,10092453,16109943,12193636,15668000,15272571,23944997